Data/hora: 10/06/2024 06:29:00

Provedor de dados: | Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
País: | Japan
Título: | Immunization with Toxoplasma gondii peroxiredoxin 1 induces protective immunity against toxoplasmosis in mice
Autores: | Fereig, Ragab M.; Kuroda, Yasuhiro; Terkawi, Mohamad Alaa; Mahmoud, Motamed Elsayed; Nishikawa, Yoshifumi.
Data: | 2017-04-27
Ano: | 2017
Resumo: | To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production. In response to TgPrx1, activation of NF-kappa B and IL-6 production were confirmed in mouse macrophage cell line (RAW 264.7). These results suggest the immune-stimulating potentials of TgPrx1. Immunization of mice with recombinant TgPrx1 stimulated specific antibody production (IgG1 and IgG2c). Moreover, spleen cell proliferation and interferon-gamma production significantly increased in the TgPrx1-sensitized cells from mice immunized with the same antigen. Immunization with TgPrx1 also increased mouse survival and decreased cerebral parasite burden against lethal T. gondii infection. Thus, our results suggest that TgPrx1 efficiently induces humoral and cellular immune responses and is useful as a new vaccine antigen against toxoplasmosis.
Idioma: | Inglês

Identificador:

http://ir.obihiro.ac.jp/dspace/handle/10322/4462

info:doi/10.1371/journal.pone.0176324

Editor: | PLOS
Fonte: | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. &lt;http://creativecommons.org/lisenses/by/4.0/&gt;