Data/hora: 10/06/2024 09:03:06

Provedor de dados: | 22
País: | Brazil

Título:

Induction and regeneration of somatic embryos of sugarcane RB996961

Indução e regeneração de embriões somáticos de cana-de-açúcar RB996961

Autores: | Almeida, Rodrigo de Oliveira; Silva, Sérgio Delmar dos Anjos e; Vargas, Daiane Peixoto; Dutra, Leonardo Ferreira.
Data: | 2015-09-18
Ano: | 2015
Palavras-chave: | Saccharum spp.; Cultura de tecido vegetal; Embriogênese somática.
Resumo: | Sugarcane is a crop widely planted in Brazil because of the economic importance of its products, like sugar and ethanol. Thus, the crop is often inserted in breeding programs. Therefor, the aim of this study was to evaluate the morphogenic response to somatic embryogenesis and somatic embryos regeneration for genotype RB996961. Transversal segments of young leave rolls with 2-3 mm size from plants with 6 to 9 months old were subcultured on solid MS medium supplemented with three concentrations of 2.4-dichlorophenoxyacetic acid (2.4-D) for embryogenic callus induction. Better results were obtained using 13.5 or 31.5 µM L-1 of 2.4-D in 45 or 60 days of incubation on induction medium, one to three subculture and 15 to 45 days of incubation on regeneration medium for genotype RB996961. This results show that the protocol used to genotype RB996961 was efficient to somatic embryos production (over 80%), nevertheless, it is necessary to adjust the regeneration approach (62.3 to 73.4%).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português

Identificador:

http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081

10.1818/sap.v14i3.9081

Editor: | Scientia Agraria Paranaensis
Relação: | http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081/8912
Formato: | application/pdf

Fonte:

Scientia Agraria Paranaensis; v. 14, n. 3 (2015); 154-159

1983-1471