Data/hora: 09/06/2024 09:27:23

Provedor de dados: | Revista Scientia Agraria Paranaensis
País: | Brazil

Título:

DNA barcoding: a molecular appliance for fish species identification support

DNA barcoding: uma ferramenta de apoio molecular para identificação de espécies de peixes

Autores: | Tresbach, Rafael Hencke; Cerqueira, Natalia Menezes; Medeiros, Sarah Ramos; Gutierrez, Harold Julián Pérez; Hernández, Natalia Ossa; Rodrigues, Marília Danyelle Nunes.
Data: | 2015-06-19
Ano: | 2015
Palavras-chave: | DNA barcoding; Peixes; Rhamdia quelen; COI; DNA mitocondrial; DNA ribossomal.
Resumo: | The identification of genus and species is made by systematic, by noticing morphological and anatomical characteres which were the phenotype expression. However, changes in one or more gene bases can result in phenotypes similarities between different species, inducing an identification error. Alternatively, molecular appliances based in mithocondrial and ribosomal genes appeared. This study aims to compile information about the molecular identification and present the South American Catfish (Rhamdia quelen) case, for which there is controversy generated by taxonomy identification. The different genes used to identification have their particularities, like insertions and deletions on the case of 16S rRNA and the 18S rRNA. Besides these ribosomal genes, mitochondrial genes are used, like the cytochrome b gene, whose problematic focused on the primer design, and the cytochrome C oxidase subunit 1 gene, which is the best option to make the identification, by generating a genetic barcode. DNA barcoding studies help on the molecular identification of species and allow evolutionary studies in special, regarding the speciation process, revealing mechanisms that may have contributed to the adaption in Brazilian different climes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português

Identificador:

http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408

10.1818/sap.v14i2.10408

Editor: | Scientia Agraria Paranaensis

Relação:

http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408/8549

http://e-revista.unioeste.br/index.php/scientiaagraria/article/downloadSuppFile/10408/1298

Formato: | application/pdf

Fonte:

Scientia Agraria Paranaensis; v. 14, n. 2 (2015); 77-81

1983-1471