Data/hora: 15/06/2024 18:18:22

Provedor de dados: 	Open Marine Archive	
País: 	Belgium	
Título: 	Application of AFLP for taxonomic and epidemiological studies of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;	
Autores: 	Thyssen, A.; Van Eygen, S.; Hauben, L.; Goris, J.; Swings, J.; Ollevier, F.P.	
Data: 	2000	
Ano: 	2000	
Palavras-chave: 	Epidemiology; Taxonomy.	
Resumo: 	A collection of 106 &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt; strains and 19 &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;damselae&lt;/i&gt; strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases &lt;i&gt;Hind&lt;/i&gt;III and &lt;i&gt;Taq&lt;/i&gt;I. Using numerical analysis, six clusters were recognized. The largest cluster (n=106) contained the majority of the strains tested and consisted exclusively of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;. The &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;damselae&lt;/i&gt; strains fell outside this cluster. DNA-DNA hybridization experiments showed 77% DNA binding between the two subspecies, indicating a close genetic relationship. This clearly demonstrates the applicability of AFLP in studying the taxonomic position of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;. In addition, AFLP proved to be a useful genotypic technique for epidemiological surveys of the pathogen, since it was able to discriminate between Mediterranean and Japanese &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt; isolates.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9527	
Fonte: 	%3Ci%3EInt.+J.+Syst.+Bacteriol.+50%3C%2Fi%3E%3A+1013-1019	
Fonte: 	info:eu-repo/semantics/restrictedAccess