Data/hora: 06/06/2024 12:03:20

Provedor de dados: | Open Marine Archive
País: | Belgium
Título: | Live-food mediated drug delivery as a tool for disease treatment in larviculture: the enrichment of therapeutics in rotifers and &lt;i&gt;Artemia&lt;/i&gt; nauplii
Autores: | Verpraet, R.; Chair, M.; Léger, P.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P.
Data: | 1992
Ano: | 1992
Palavras-chave: | Aquaculture systems; Disease control; Drugs; Food chains; Therapy; Artemia.
Resumo: | The live food enrichment technique using emulsions for nutritional deficiencies in rotifers and &lt;i&gt;Artemia&lt;/i&gt; was further investigated as a tool for transferring therapeutics through the food chain. &lt;i&gt;Artemia&lt;/i&gt; nauplii and the rotifer &lt;i&gt;Brachionus plicatilis&lt;/i&gt; were enriched with a self-emulsifying concentrate in which the therapeutic mixture trimetoprim: sulfamethoxazole was incorporated. An analytical method and protocol for the quantification of trimethoprim and sulfamethoxazole in a heterogeneous biological matrix, such as &lt;i&gt;Artemia&lt;/i&gt; , was developed using High Performance Liquid Chromatography. When applying a 24h enrichment period, the accumulation of both drugs in &lt;i&gt;Artemia&lt;/i&gt; nauplii appeared to be linearly related to the concentration of the drugs in the enrichment product. Values of approximately 790 mg/kg of total drugs (protein basis; or 290 mg/kg on dry weight basis) could be obtained. In rotifers, the level of total therapeutics reached a value of about 116,mg/kg (protein basis) after 6h of enrichment. The ratio of the two drugs detected in rotifers was different from the one found in &lt;i&gt;Artemia&lt;/i&gt; , suggesting different accumulation kinetics.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2894
Fonte: | %3Ci%3EAquacult.+Eng.+11%3C%2Fi%3E%3A+133-139
Fonte: | info:eu-repo/semantics/restrictedAccess