Data/hora: 07/06/2024 09:26:23

Provedor de dados: | Open Marine Archive
País: | Belgium
Título: | Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (&lt;i&gt;Artemia&lt;/i&gt; spp.) used for prophylactic chemotherapy of fish
Autores: | Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P.
Data: | 1991
Ano: | 1991
Palavras-chave: | Aquaculture techniques; Fish culture; Infectious diseases; Medicine; Artemia.
Resumo: | The brine shrimp &lt;i&gt;Artemia&lt;/i&gt; , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in &lt;i&gt;&lt;i&gt;Artemia&lt;/i&gt; franciscana&lt;/i&gt;, a sensitive and specific analytical method was developed. It is based on homogenization of &lt;i&gt;Artemia&lt;/i&gt; nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C&lt;sub&gt;18&lt;/sub&gt; cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in &lt;i&gt;A. franciscana&lt;/i&gt;, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/imisdocs/publications/262329.pdf
Formato: | application/pdf
Fonte: | %3Ci%3EAntimicrob.+Agents+Chemother.+35%2812%29%3C%2Fi%3E%3A+2486-2489
Fonte: | info:eu-repo/semantics/openAccess