Data/hora: 03/06/2024 16:55:11

Provedor de dados: | 27
País: | Belgium
Título: | The effect of supplemental ascorbic acid in enriched live food for &lt;i&gt;Clarias gariepinus&lt;/i&gt; larvae at startfeeding
Autores: | Merchie, G.; Lavens, P.; Verreth, J.; Ollevier, F.P.; Nelis, H.; De Leenheer, A.P.; Storch, V.; Sorgeloos, P.
Data: | 1997
Ano: | 1997
Palavras-chave: | Fish larvae; Food organisms; Vitamin C; Clarias gariepinus.
Resumo: | The effect of three dietary ascorbic acid (AA) concentrations, each applied via two feed types, on production characteristics and physiological condition of African catfish (&lt;i&gt;Clarius gariepinus&lt;/i&gt;) larvae has been assessed in two 10-day culture trials. Three treatments received only &lt;i&gt;Artemia&lt;/i&gt; nauplii enriched with an experimental emulsion containing 0, 10 or 20% ascorbyl palmitate (AP) and yielding 530, 1200 and 1600 µg AA g&lt;sup&gt;-1&lt;/sup&gt; DW &lt;i&gt;Artemia&lt;/i&gt; , respectively; the other three treatments were fed the same &lt;i&gt;Artemia&lt;/i&gt; diets which were partially substituted by an artificial diet containing no vitamin C (ratio 20:80). No differences in survival could be observed: however, from day 6 onwards the 20%-AP group showed significantly better growth compared to the 0%- and 10%- treatments. For the cofeeding series, the same positive, but not significant, influence of vitamin C on dry weight was found. Moreover, the animals receiving the highest vitamin C supplementation displayed a considerably lower stress sensitivity than those of the 0%- and the 10%-AP groups, for both the 100%-and the 20%- &lt;i&gt;Artemia&lt;/i&gt; series. These differences had occurred by day 2, which might be indicative of the importance of AA in early development. A second trial, which was a repetition of the first one, revealed the same tendencies; however, growth differences were smaller, probably due to the higher incorporation levels of AA obtained in the live diet (530, 1700 and 2300 µg AA g &lt;sup&gt;-1&lt;/sup&gt; DW) and in the catfish larvae. Growth results of both experiments were supported with data from the ultrastructural evaluation of the hepatocytes: i.e. a more organized cell compartmentation and better-structured cell organelles in the 20%-AP group of the &lt;i&gt;Artemia&lt;/i&gt; series compared to the control are indicative of a more active metabolism. The slow growth in the cofeeding series was documented by the poor condition of the hepatocytes.In a third experiment it was verified that the growth effect of the 20%-AP boosted &lt;i&gt;Artemia&lt;/i&gt; diet was the result of the extra AA incorporation and not of the concomitant palmitic acid (PA), which was set free after hydrolysis of AP in the &lt;i&gt;Artemia&lt;/i&gt; nauplii, and which could possibly be used as a supplemental energy source. The three treatments were fed &lt;i&gt;Artemia&lt;/i&gt; nauplii enriched with 0% AP, 12% PA and 20% AP, respectively. Growth and stress resistance of the latter group were significantly higher compared to the control and the PA-supplemented fish. To our knowledge this is the first evidence for the positive role of high dietary vitamin C levels (more than 1500 µg AA g&lt;sup&gt;-1&lt;/sup&gt; DW) on larval development of an aquaculture species, and more specifically of &lt;i&gt;C. gariepinus&lt;/i&gt;.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2963
Fonte: | %3Ci%3EAquaculture+151%3C%2Fi%3E%3A+245-258
Fonte: | info:eu-repo/semantics/restrictedAccess