Data/hora: 10/06/2024 08:03:17

Provedor de dados: 	Acta Scientiarum: Biological Sciences	
País: 	Brazil	
Título: 	Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777	
Autores: 	Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Troni, Allan Reis; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Takamura, Angela Emi; Universidade Federal de Viçosa.	
Data: 	2012-12-18	
Ano: 	2012	
Palavras-chave: 	2.04.06.00-2; 5.05.04.00-2; 5.06.03.00-0 creole frog; GnRHa; Semen; Spermatozoon; Sperm motility Aquicultura.	
Resumo: 	By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate.  	
Tipo: 	Estudos morfológicos	
Idioma: 	Inglês	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777

10.4025/actascibiolsci.v35i1.14777	
Editor: 	Universidade Estadual De Maringá	
Formato: 	application/pdf

application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 35, n. 1 (2013); 1-7

Acta Scientiarum. Biological Sciences; v. 35, n. 1 (2013); 1-7	
Fonte: 	DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).

DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).