Data/hora: 03/06/2024 06:26:40

Provedor de dados: | Acta Scientiarum: Biological Sciences
País: | Brazil
Título: | Antibacterial activity of different types of snake venom from the Viperidae family against Staphylococcus aureus
Autores: | Canhas, Isabela Nascimento; Heneine, Luiz Guilherme Dias; Fraga, Thaís; Assis, Débora Cristina Sampaio de; Borges, Márcia Helena; Chartone-Souza, Edmar; Nascimento, Andréa Maria Amaral.
Data: | 2017-09-18
Ano: | 2017
Palavras-chave: | Bothrops moojeni; Bacteria; Antibiotic-resistance; NorA efflux pump; Biofilm.
Resumo: | Toxins and venoms produced by living organisms have exhibited a variety of biological activities against microorganisms. In this study, we tested seven snake venoms from the family Viperidae for antibacterial activity and the activities of reversal of antibiotic resistance and inhibition of biofilm formation against 22 clinical isolates of Staphylococcus aureus. Bothrops moojeni venom exhibited anti staphylococcal activity with the lowest mean value of minimum inhibitory concentration (MIC). Moreover, reversal of antibiotic resistance was observed for combinations of B. moojeni venom (½ x MIC) and norfloxacin or ampicillin (both ½ x MIC) for 86.4% and 50% of the isolates, respectively. B. moojeni venom alone at ½ MIC inhibited 90% of biofilm formation, whereas in combination with ciprofloxacin, both at ½ MIC, a reduction on the NorA efflux pump activity was observed. The detection of in vitro mutants colonies of S. aureus resistant to B. moojeni venom was low and they did not survive. A phospholipase A2 was purified from the venom of B. moojeni and displayed anti-staphylococcal activity when tested alone or in combination with ciprofloxacin. The results presented here will contribute to the search for new antimicrobial agents against resistant S. aureus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33826

10.4025/actascibiolsci.v39i3.33826

Editor: | Universidade Estadual De Maringá

Relação:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33826/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/33826/13493

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/33826/13494

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/33826/13497

Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 39, n. 3 (2017); 309-319

Acta Scientiarum. Biological Sciences; v. 39, n. 3 (2017); 309-319

1807-863X

1679-9283

Fonte: | Direitos autorais 2017 Acta Scientiarum. Biological Sciences