Data/hora: 06/06/2024 01:39:37

Provedor de dados: 	3	
País: 	Brazil	
Título: 	Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products - doi: 10.4025/actascibiolsci.v32i3.1519	
Autores: 	Borsari, Raquel Renan Jorge; Universidade Estadual de Londrina; Buzato, João Batista; UEL; Silva, Rui Sérgio dos Santos Ferreira da; UEL; Celligoi, Maria Antonia Pedrini Colabone; UEL.	
Data: 	2010-09-15	
Ano: 	2010	
Palavras-chave: 	2.08.02.00-5; 2.12.02.02-8 Zymomonas mobilis; Fermentation; Biomass; Substrate; Sorbitol; Levan Bioquímica dos microrganismos; Microbiologia industrial e de fermentação Zymomonas mobilis; Fermentation; Biomass; Substrate; Sorbitol; Levan.	
Resumo: 	The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.	
Idioma: 	Inglês	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1519

10.4025/actascibiolsci.v32i3.1519	
Editor: 	Universidade Estadual De Maringá	
Formato: 	application/pdf

application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 32, n. 3 (2010); 229-233

Acta Scientiarum. Biological Sciences; v. 32, n. 3 (2010); 229-233	
Fonte: 	DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).

DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).