Data/hora: 12/06/2024 09:52:07

Provedor de dados: 	3	
País: 	Brazil	
Título: 	Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification	
Autores: 	Prudente, Débora de Oliveira; Paiva, Renato; Nery, Fernanda Carlota; Máximo, Wesley Pires Flausino; Silva, Luciano Coutinho.	
Data: 	2017-05-03	
Ano: 	2017	
Palavras-chave: 	Botânica  criopreservação de recursos genéticos vegetais Mangabeira; Recalcitrant species; Long-term conservation; Dehydration. Botânica.	
Resumo: 	 Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. 	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620

10.4025/actascibiolsci.v39i1.31620	
Editor: 	Universidade Estadual De Maringá	
Relação: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12232

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12233

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12234

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12235

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12236

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14141

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14142

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14143

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14144	
Formato: 	application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 39, n. 1 (2017); 87-93

Acta Scientiarum. Biological Sciences; v. 39, n. 1 (2017); 87-93

1807-863X

1679-9283	
Fonte: 	Direitos autorais 2017 Acta Scientiarum. Biological Sciences