Data/hora: 03/06/2024 18:03:44

Provedor de dados: 	3	
País: 	Brazil	
Título: 	Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture	
Autores: 	Gomes, Lucas Roberto Pereira; Franceschi, Cristina do Rosário Batista; Ribas, Luciana Lopes Fortes.	
Data: 	2015-08-06	
Ano: 	2015	
Palavras-chave: 	Biotecnologia; Botânica epiphytic orchid; In vitro propagation; Protocorms; Protocorm-like body 2.03.00.00-0.	
Resumo: 	An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival). 	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276

10.4025/actascibiolsci.v37i2.27276	
Editor: 	Universidade Estadual De Maringá	
Relação: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276/pdf_49	
Formato: 	application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149

Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149

1807-863X

1679-9283