Data/hora: 11/06/2024 03:29:44

Provedor de dados: | Acta Scientiarum: Biological Sciences
País: | Brazil
Título: | Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.
Autores: | Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
Data: | 2015-03-10
Ano: | 2015
Palavras-chave: | Tecnolologia de alimentos  citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Resumo: | This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p &lt; 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p &lt; 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119

10.4025/actascibiolsci.v37i1.23119

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119/pdf_42
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 37, n. 1 (2015); 101-106

Acta Scientiarum. Biological Sciences; v. 37, n. 1 (2015); 101-106

1807-863X

1679-9283