Data/hora: 04/06/2024 02:45:35

Provedor de dados: | 3
País: | Brazil
Título: | An anti-dengue and anti-herpetic polysulfated fraction isolated from the coenocytic green seaweed Caulerpa cupressoides inhibits thrombin generation in vitro
Autores: | Rodrigues, José Ariévilo Gurgel; Eloy, Ygor Raphael Gomes; Vanderlei, Edfranck de Souza Oliveira; Cavalcante, Jéssica Figueiredo; Romanos, Maria Tereza Villela; Benevides, Norma Maria Barros.
Data: | 2017-06-16
Ano: | 2017
Palavras-chave: | Química de macromoléculas Bryopsidales; Marine glycans; Cytotoxicity; Viral infection; Thrombin. Bioprospecção.
Resumo: | The antiviral potency of Caulerpa cupressoides (Chlorophyta) sulfated polysaccharidic fractions (Cc-SP1→3) associated with thrombin generation (TG) assay is unknown. This study analyzed the structure of Cc-SP1 and its effects against herpes viruses (HSV-1 and HSV-2), dengue-virus (DENV-1), human-metapneumovirus (HMPV) and adenovirus (AdV) in Vero and mosquito C6/36 cell-lines and as inhibitor of TG in 60-fold diluted human plasma using continuous system. Infrared analysis indicated ulvan containing 11% sulfate, 40.16% total sugars and 6% uronic acid. Procedures of agarose/polyacrylamide gels electrophoresis revealed two major components presenting sulfate and non SPs, with molecular dispersion ranging from 8 to &gt; 100 kDa, respectively, as confirmed by gel permeation chromatography. Cc-SP1 did not cause cytotoxicity up to 1000 μg mL-1 and was more effective inhibitor of DENV-1 (96%, 0.35 μg mL-1) compared with HSV-1 (90%, 27 μg mL-1) and HSV-2 (99.9%, 0.9 μg mL-1) in Vero cell-line, whose selectivity indexes were &gt; 714, &gt; 9.2 and &gt; 131, respectively, but was inactive against HMPV, AdV and C6/36 cell-line using plate reduction assay. Cc-SP1 required concentration 20.8-fold higher of SPs than heparin for abolishes intrinsically TG, but at concentrations so far the anti-HSV-1. Therefore, TG assay provides data to guide studies of Cc-SP1 on anticoagulant/antiviral effects.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081

10.4025/actascibiolsci.v39i2.28081

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081/pdf
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 149-159

Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 149-159

1807-863X

1679-9283

Fonte: | Direitos autorais 2017 Acta Scientiarum. Biological Sciences