Data/hora: 09/06/2024 04:20:33

Provedor de dados: | Acta Scientiarum: Biological Sciences
País: | Brazil

Título:

Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788

Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788

Autores: | Nascente, Patrícia da Silva; Universidade Federal de pelotas; Coimbra, Helen Silveira; Universidade Federal de Pelotas; Meinerz, Ana Raquel Mano; Universidade Federal de Pelotas; Meireles, Mário Carlos Araújo; Universidade Federal de Pelotas; Mello, João Roberto Braga; Universidade Federal do Rio Grande do Sul; Schuch, Luiz Filipe Damé; Universidade Federal de Pelotas.
Data: | 2010-05-28
Ano: | 2010
Palavras-chave: | 2.00.00.00-6; 2.08.04.00-8; 2.12.00.00-9; 2.12.01.03-0 Malassezia pachydermatis; Molecular biology; Otitis externa; Dogs Ciências Biológicas; Biologia Molecular; Microbiologia; Micologia Malassezia pachydermatis; Molecular biology; Otitis externa; Dogs.
Resumo: | Several methodologies in molecular biology have been used in the investigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.
Tipo: | Estudo molecular
Idioma: | Português

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4788

10.4025/actascibiolsci.v32i2.4788

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 32, n. 2 (2010); 169-173

Acta Scientiarum. Biological Sciences; v. 32, n. 2 (2010); 169-173

Fonte:

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DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).