Data/hora: 09/06/2024 11:35:35

Provedor de dados: | Acta Amazonica
País: | Brazil
Título: | Transferability of heterologous primers in Brycon falcatus
Autores: | PENHA,Diego dos Santos; SOUZA,Felipe Pinheiro de; LIMA,Ed Christian Suzuki de; URREA-ROJAS,Angela Maria; PANDOLFI,Victor César Freitas; YAMACHITA,Andrei Lincoln; POVH,Jayme Aparecido; LEITE,Natalia Gonçalves; PEREIRA,Ulisses de Pádua; LOPERA-BARRERO,Nelson Mauricio.
Data: | 2020-09-01
Ano: | 2020
Palavras-chave: | Bryconidae; Cross amplification; Genetic conservation; Genetic variability; SSR.
Resumo: | ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232
Editor: | Instituto Nacional de Pesquisas da Amazônia
Relação: | 10.1590/1809-4392201904191
Formato: | text/html
Fonte: | Acta Amazonica  v.50 n.3 2020
Fonte: | info:eu-repo/semantics/openAccess