Data/hora: 10/06/2024 14:05:43

Provedor de dados: 	Acta Amazonica	
País: 	Brazil	
Título: 	A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment	
Autores: 	MANGARAVITE,Érica; TERRA,Vanessa; HATTORI,Eric Koiti Okiyama; DAL’SASSO,Thaís Carolina da Silva; BHERING,Leonardo Lopes; OLIVEIRA,Luiz Orlando de.	
Data: 	2020-12-01	
Ano: 	2020	
Palavras-chave: 	Ascorbic acid; Dithiothreitol; DNA isolation.	
Resumo: 	ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.	
Tipo: 	Info:eu-repo/semantics/report	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335	
Editor: 	Instituto Nacional de Pesquisas da Amazônia	
Relação: 	10.1590/1809-4392202001571	
Formato: 	text/html	
Fonte: 	Acta Amazonica  v.50 n.4 2020	
Fonte: 	info:eu-repo/semantics/openAccess