Data/hora: 01/06/2024 06:35:44

Provedor de dados: | Acta Amazonica
País: | Brazil
Título: | Evaluation of antimutagenic and cytotoxic activity of skin secretion extract of Rhinella marina and Rhaebo guttatus (Anura, Bufonidae)
Autores: | OLIVEIRA,Angellica Fernandes de; CASTOLDI,Lindsey; VIEIRA JUNIOR,Gerardo Magela; MONÇÃO FILHO,Evaldo dos Santos; CHAVES,Mariana Helena; RODRIGUES,Domingos de Jesus; SUGUI,Marina Mariko.
Data: | 2019-04-01
Ano: | 2019
Palavras-chave: | Genotoxicity; Bioprospecting; Micronuclei; Chemoprevention.
Resumo: | ABSTRACT The skin secretion from toads of the Bufonidae family has great potential in the search for new active compounds to be used as drug candidates in treating some diseases, among them cancer. In this context, this study aimed to evaluate the cytotoxic and antimutagenic activity of the parotoid gland secretion extracts of Rhinella marina and Rhaebo guttatus, as well as biochemically analyze transaminases and serum creatinine for liver and renal damage, respectively. Cytotoxicity was performed by the colorimetric method based on MTT (3- [4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide) with different concentrations of the extracts in Walker or splenic tumor cell cultures from rats and mice. The micronucleus test was performed with male Swiss mice treated orally with the extracts for 15 days, and then intraperitoneally with N-ethyl-N-nitrosurea (50 mg kg-1). Micronucleated polychromatic erythrocytes (MNPCE) were evaluated in bone marrow. The extracts showed cytotoxic activity in the evaluated cells. There was a significant reduction in the frequency of MNPCE (R. marina = 56% and R. guttatus = 75%, p &lt; 0.001), indicating antimutagenic potential of the extracts. The groups treated only with extract showed an increase in MNPCE frequency, evidencing mutagenic potential. Biochemical analyzes showed no significant difference between treatments. Thus, under our experimental conditions, the extracts of R. marina and R. guttatus skin secretions presented chemopreventive potential for cancer.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000200145
Editor: | Instituto Nacional de Pesquisas da Amazônia
Relação: | 10.1590/1809-4392201801751
Formato: | text/html
Fonte: | Acta Amazonica  v.49 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess