Data/hora: 09/06/2024 05:35:14

Provedor de dados: | Acta Biológica Colombiana
País: | Colombia
Título: | ISOLATION OF THE HUMAN CYTOMEGALOVIRUS FROM BODILY FLUIDS
Autores: | CAMACHO ORTEGA,Sigrid Johanna; BOHORQUEZ ÁVILA,Sonia del Pilar; VELANDIA ROMERO,Myriam Lucia; CASTELLANOS PARRA,Jaime Eduardo.
Data: | 2019-12-01
Ano: | 2019
Palavras-chave: | Body fluids; Cell culture; Cytomegalovirus; Isolation.
Resumo: | ABSTRACT In vitro studies on the pathogenesis of the human cytomegalovirus (HCMV) are conducted regularly using laboratory adapted strains that lose some characteristics during the adaptation process. Since HCMV is excreted from bodily fluids during infection or reactivation, this work aimed to isolate and culture HCMV from the MRC-5 human cells found in the urine, bronchoalveolar lavage, saliva, and plasma samples of pediatric patients with probable or confirmed infection. The samples were inoculated on cell cultures either for 14 days or until a cytopathic effect (CPE) of 80 % was observed. The cell lysates and supernatants were used to perform successive viral passages. Besides HCMV, the herpes simplex virus was detected from all the saliva samples. Inoculation of the HCMV positive sera induced cell clustering and immediate monolayer damage that restricted their use. One sample of bronchoalveolar lavage induced a CPE after inoculation like that of the HCMV reference strains (Towne and Merlin), which was consequently propagated and titrated. A second viral isolate derived from the urine sample of a patient with congenital infection did not demonstrate a CPE, although presence of the virus had been confirmed using PCR. The viral isolates were examined and found to be negative for adenoviruses or enteroviruses. Despite the evident difficulty encountered for the isolation and harvesting of the HCMV, this work shows that it was possible to obtain a low passage viral strain using a modified shell vial method and inoculation protocol with extended follow-up and confirmation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300520
Editor: | Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
Relação: | 10.15446/abc.v24n3.79406
Formato: | text/html
Fonte: | Acta Biológica Colombiana  v.24 n.3 2019
Fonte: | info:eu-repo/semantics/openAccess