Data/hora: 10/06/2024 15:20:34

Provedor de dados: 	39	
País: 	Brazil	
Título: 	In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)	
Autores: 	Balbuena,Tiago Santana; Santa-Catarina,Claudete; Silveira,Vanildo; Kato,Massuo Jorge; Floh,Eny Iochevet Segal.	
Data: 	2009-03-01	
Ano: 	2009	
Palavras-chave: 	Callogenesis; Cell suspension culture; Morphogenesis; Pariparoba; Plant growth regulators.	
Resumo: 	Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100029	
Editor: 	Sociedade Botânica do Brasil	
Relação: 	10.1590/S0102-33062009000100029	
Formato: 	text/html	
Fonte: 	Acta Botanica Brasilica  v.23 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess