Data/hora: 09/06/2024 09:31:25

Provedor de dados: | Acta Botanica Brasilica
País: | Brazil
Título: | Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp.
Autores: | Pereira,Marlon Corrêa; Rocha,Diego Ismael; Veloso,Tomás Gomes Reis; Pereira,Olinto Liparini; Francino,Dayana Maria Teodoro; Meira,Renata Maria Strozi Alves; Kasuya,Maria Catarina Megumi.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Ceratorhiza; Epulorhiza; Orchid conservation; Seedling production; Symbiosis.
Resumo: | Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until leaf production at 63 days. The protocorms are comprised of parenchyma cells delimited by a unistratified epidermis; the parenchyma cells of the upper part of the protocorms are smaller than those located more towards the base. Intact and digested pelotons were observed inside of protocorms implying that the seedlings were capable of mycotrophy. Additionally, the development of a bud primordium only occurred after colonization by fungus. This study suggests that C. glutiniferum has a preference for strains of Epulorhiza and that fungus digestion is essential to protocorm development.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567
Editor: | Sociedade Botânica do Brasil
Relação: | 10.1590/0102-33062015abb0078
Formato: | text/html
Fonte: | Acta Botanica Brasilica  v.29 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess