Data/hora: 11/06/2024 01:05:40

Provedor de dados: | 42
País: | Brazil
Título: | High susceptibility of activated lymphocytes to oxidative stress-induced cell death
Autores: | Degasperi,Giovanna R.; Castilho,Roger F.; Vercesi,Anibal E.
Data: | 2008-03-01
Ano: | 2008
Palavras-chave: | Cell death; Free radicals; Immune response; Lymphopenia; Mitochondrial permeability transition; Spleen lymphocyte.
Resumo: | The present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in activated lymphocytes increases their susceptibility to oxidative stress-induced cell death in a mechanism involving the participation of mitochondrial permeability transition.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100009
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652008000100009
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.80 n.1 2008
Fonte: | info:eu-repo/semantics/openAccess