Data/hora: 16/06/2024 11:09:00

Provedor de dados: | 42
País: | Brazil
Título: | Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
Autores: | FELICIO,ANA LUCIA S.M.; MONTEIRO,ALESSANDRA M.; ALMEIDA,MARIANA B.; MADEIRA,TIAGO B.; NIXDORF,SUZANA L.; YABE,MARIA JOSEFA S.
Data: | 2016-09-01
Ano: | 2016
Palavras-chave: | Pesticides; Degradation products; HPLC-UV; Soil; Environmental control.
Resumo: | ABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201620150234
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.88 n.3 2016
Fonte: | info:eu-repo/semantics/openAccess