Data/hora: 09/06/2024 04:48:00

Provedor de dados: 	Anais da Academia Brasileira de Ciências	
País: 	Brazil	
Título: 	Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS	
Autores: 	SOUZA,JOANNE M.M.; BERKOV,STRAHILL; SANTOS,ALBERDAN S.	
Data: 	2014-09-01	
Ano: 	2014	
Palavras-chave: 	Anti-inflammatory lipids; Boerhaavia paniculata; Callus culture; Lipid profile; Medicinal plant.	
Resumo: 	In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201420130098	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.86 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess