Data/hora: 10/06/2024 17:06:39

Provedor de dados: | Anais da Academia Brasileira de Ciências
País: | Brazil
Título: | Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells
Autores: | Schwindt,Telma T.; Motta,Fabiana L.; Gabriela F.,Barnabé; Cristina G.,Massant; Guimarães,Alessander O.; Calcagnotto,Maria Elisa; Pesquero,João B.; Mello,Luiz E.
Data: | 2009-09-01
Ano: | 2009
Palavras-chave: | Neural precursor cells; Neurosphere; Fibroblast growth factor 2; Epidermal growth factor; Differentiation.
Resumo: | Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652009000300009
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.81 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess