Data/hora: 05/06/2024 07:59:35

Provedor de dados: | Anais da Academia Brasileira de Ciências
País: | Brazil
Título: | Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts
Autores: | PINTO,NÍCOLAS C.C.; SILVA,JUCÉLIA B.; MENEGATI,LAURA M.; GUEDES,MARIA CLARA M.R.; MARQUES,LUCAS B.; SILVA,THIAGO P. DA; MELO,ROSSANA C.N. DE; SOUZA-FAGUNDES,ELAINE M. DE; SALVADOR,MARCOS J.; SCIO,ELITA; FABRI,RODRIGO L.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Alkaloids; Annona squamosa; Antibacterial; Citotoxicity; Herbal medicine.
Resumo: | ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720150702
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.3 suppl.0 2017
Fonte: | info:eu-repo/semantics/openAccess