Data/hora: 16/06/2024 16:28:16

Provedor de dados: 	Anais da Academia Brasileira de Ciências	
País: 	Brazil	
Título: 	Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection	
Autores: 	Pereira,Wânia F.; Guillermo,Landi V.C.; Ribeiro-Gomes,Flávia L.; Lopes,Marcela F.	
Data: 	2008-03-01	
Ano: 	2008	
Palavras-chave: 	Apoptosis; Caspase-8; CD8 T cells; IFN-gamma; Leishmaniasis.	
Resumo: 	Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following Tcellactivation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-FasLigand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile- Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-gamma expression in both CD4 and CD8T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediated type-1 responses during L. major infection.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100008	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/S0001-37652008000100008	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.80 n.1 2008	
Fonte: 	info:eu-repo/semantics/openAccess