Data/hora: 04/06/2024 01:01:27

Provedor de dados: | 42
País: | Brazil
Título: | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
Autores: | Mondino,Eduardo A.; Covacevich,Fernanda; Studdert,Guillermo A.; Pimentel,João P.; Berbara,Ricardo L.L.
Data: | 2015-06-01
Ano: | 2015
Palavras-chave: | Agroecosystems; Nematodes; Nucleic acid extraction; CTAB.
Resumo: | We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201520130110
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.87 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess