Data/hora: 08/06/2024 11:59:48

Provedor de dados: 	Anais da Academia Brasileira de Ciências	
País: 	Brazil	
Título: 	High-fat diet and streptozotocin in the induction of type 2 diabetes mellitus: a new proposal	
Autores: 	MAGALHÃES,DIEGO A. DE; KUME,WILLIAN T.; CORREIA,FRANCYELE S.; QUEIROZ,THAÍS S.; ALLEBRANDT NETO,EDGAR W.; SANTOS,MAÍSA P. DOS; KAWASHITA,NAIR H.; FRANÇA,SUÉLEM A. DE.	
Data: 	2019-01-01	
Ano: 	2019	
Palavras-chave: 	Diabetes mellitus type 2 model; High-fat diet; Streptozotocin; Rats.	
Resumo: 	Our objective was to establish a diabetes mellitus type 2 (DM2) model in rats using a high-fat diet and streptozotocin (HF-STZ). Male Wistar rats (240-250g) were divided into a control group (commercial feed), and HF-STZ group, (66.5%-commercial feed, 13.5%-lard, and 20%-sugar). STZ (40mg/kg i.p.) or vehicle was administered on the 13th day. An oral glucose tolerance test (OGTT) was performed (2.5mg of glucose/kg v.o.) on both groups. After 39 days of treatment, blood and tissue samples were collected for analyses. The weight gain after STZ administration was lower in the HF-STZ group than in the control group with reductions in muscle mass and adipose tissue. The HF-STZ group showed hyperglycemia after STZ administration (glucose on day 39: HF-STZ: 499 ± 60; control: 134 ± 9mg/dL). Serum glucagon was 23% lower, and insulin levels were unaltered. The HOMA index was 4-times higher in the HF-STZ. The HF-STZ group showed increased post-prandial (330%) and fasting (125%) triglycerides, and while glycogen content in the liver and muscles decreased (70-80%). The area under the curve (OGTT) was 282% higher in the HF-STZ group. The combination of high-fat diet with STZ (i.p) generated rats with hyperglycemia associated with hypertriglyceridemia and introduced many other alterations present in human DM2.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100615	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201920180314	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.91 n.1 2019	
Fonte: 	info:eu-repo/semantics/openAccess