Data/hora: 16/06/2024 06:55:04

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Evaluation of the photodynamic activity of Xanthene Dyes on Artemia salina described by chemometric approaches	
Autores: 	Pellosi,Diogo S.; Batistela,Vagner R.; Souza,Vagner R. de; Scarminio,Ieda S.; Caetano,Wilker; Hioka,Noboru.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Artemia salina; Factorial design; Photodynamic therapy; Bioassay; Xanthenes.	
Resumo: 	The development of drugs for photodynamic therapy (PDT) is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y) with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i) photosensitizers concentration, (ii) the LED irradiation time and (iii) the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was employed a 23 full factorial design. The death of A. salina was related to dye structure and the interaction between the irradiation time and the photosensitizers concentration. About 60% of crustaceans death was obtained using rose bengal B, which presentes the highest quantum yield of singlet oxygen due to the number of iodide substituents in the xanthenes ring. The proposed bioassay using A. salina, xanthene dyes and LED irradiation was found suitable for quantitative PDT drug evaluation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401267	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201395412	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.85 n.4 2013	
Fonte: 	info:eu-repo/semantics/openAccess