Data/hora: 08/06/2024 16:25:31

Provedor de dados: 	42	
País: 	Brazil	
Título: 	The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos	
Autores: 	CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; ALEXANDRE,RODRIGO S.	
Data: 	2018-12-01	
Ano: 	2018	
Palavras-chave: 	Carica papaya L; Malt extract; Myo-inositol; Polyethylene glycol.	
Resumo: 	Abstract This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary somatic embryos produced in experiment 2 were transferred to the germination medium (GM). The GM consisted of full-strength MS medium, sucrose, agar and was supplemented with myo-inositol at varying concentrations (0; 0.275; 0.55 and 0.825 mM). The PEG concentrations tested impaired the maturation of ‘Golden THB’ papaya somatic embryos. The MM, supplemented with malt extract at 0.153 g L-1, promoted the greatest development of normal somatic embryos (18.28 SE calli-1), that is, two cotyledonary leaves produced 36.56 SE calli-1. The supplementation with 0.45 mM myo-inositol provided the highest germination percentage (47.42%) and conversion to emblings.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703433	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201820171035	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.90 n.4 2018	
Fonte: 	info:eu-repo/semantics/openAccess