Data/hora: 09/06/2024 06:42:24

Provedor de dados: | Anais da Academia Brasileira de Ciências
País: | Brazil
Título: | Characterization and bioactivities of a novel polysaccharide obtained from Gracilariopsis lemaneiformis
Autores: | SHI,CHEN-SHAN; SANG,YA-XIN; SUN,GUI-QING; LI,TIAN-YE; GONG,ZHENG-SI; WANG,XIANG-HONG.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | Bioactivities; Gracilariopsis lemaneiformis; Polysaccharide; Structural characterization.
Resumo: | ABSTRACT Gracilariopsis lemaneiformis is a type of red alga that contains seaweed polysaccharide agar. In this study, a novel non-agar seaweed polysaccharide fraction named GCP (short of crude polysaccharide obtained from Gracilariopsis lemaneiformis) was isolated from Gracilariopsis lemaneiformis. Structural analysis showed that GCP shows triple helical chain conformation when dissolved in water and has many branches and long side chains. Also, 1→3 linkage is the major linkage and the sugar structures are galactopyranose configurations linked by β-type glycosidic linkages. Two macromolecular substance fractions (GCP-1 and GCP-2) were purified by DEAE Sepharose Fast Flow column chromatography. Moreover, a splenocyte damage assay and splenocyte proliferation assay were used to analyse the bioactivities of GCP, GCP-1 and GCP-2. It was demonstrated that polysaccharides could protect splenocyte damaged by H2O2; GCP-2 shows a greatest protection rate, that is, 92.8%, which significantly enhanced the splenocyte proliferation, and GCP showed the highest proliferation rate, 9.30%. The results suggested that this type of novel non-agar polysaccharide displayed remarkable antioxidant and immunomodulatory activities and early alkali treatment could decrease the activities. It may represent a potential material for health food and clinical medicines.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100175
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720160488
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess