Data/hora: 10/06/2024 14:53:42

Provedor de dados: | Anais da Academia Brasileira de Ciências
País: | Brazil
Título: | Bioprospecting of lipolytic microorganisms obtained from industrial effluents
Autores: | PEIL,GREICE H.S.; KUSS,ANELISE V.; RAVE,ANDRÉS F.G.; VILLARREAL,JOSÉ P.V.; HERNANDES,YOHANA M.L.; NASCENTE,PATRÍCIA S.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Effluent; Enzyme activity; Extracellular lipase production; Lipase enzyme; Lipolytic microorganism.
Resumo: | ABSTRACT The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501769
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201620150550
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.88 n.3 suppl.0 2016
Fonte: | info:eu-repo/semantics/openAccess