Data/hora: 11/06/2024 03:55:56

Provedor de dados: | 42
País: | Brazil
Título: | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
Autores: | BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | Cytotoxicity; Gas Chromatography; Phoradendron microphyllum; Phoradendron mucronatum.
Resumo: | ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720160586
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess