Data/hora: 08/06/2024 04:29:44

Provedor de dados: 	Anais da Academia Brasileira de Ciências	
País: 	Brazil	
Título: 	Biochemical and histological characterization of tomato mutants	
Autores: 	Monteiro,Carolina C.; Rolão,Milca B.; Franco,Mônica R.; Peters,Leila P.; Cia,Mariana C.; Capaldi,Flávia R.; Carvalho,Rogério F.; Gratão,Priscila L.; Rossi,Mônica L.; Martinelli,Adriana P.; Peres,Lázaro E.P.; Azevedo,Ricardo A.	
Data: 	2012-06-01	
Ano: 	2012	
Palavras-chave: 	Antioxidant enzymes; Leaf and root anatomy; Oxidative stress; Solanum lycopersicum.	
Resumo: 	Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H2O2) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE analysis, the activities of glutathione reductase (GR) isoforms III, IV, V and VI were increased in l leaves, while the activity of superoxide dismutase (SOD) isoform III was reduced in leaves of sit, epi, Never ripe (Nr) and green flesh (gf) mutants. Microscopic analyses revealed that hp1 and au showed an increase in leaf intercellular spaces, whereas sit exhibited a decrease. The au and hp1 mutants also exhibited a decreased in the number of leaf trichomes. The characterization of these mutants is essential for their future use in plant development and ecophysiology studies, such as abiotic and biotic stresses on the oxidative metabolism.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200025	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/S0001-37652012005000022	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.84 n.2 2012	
Fonte: 	info:eu-repo/semantics/openAccess