Data/hora: 11/06/2024 04:44:24

Provedor de dados: 	Anais da Academia Brasileira de Ciências	
País: 	Brazil	
Título: 	DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents	
Autores: 	AQUINO,TATIANE DE; ZENKNER,FERNANDA F.; ELLWANGER,JOEL H.; PRÁ,DANIEL; RIEGER,ALEXANDRE.	
Data: 	2016-03-01	
Ano: 	2016	
Palavras-chave: 	DNA damage; Micronucleus; Toxicology; Occupational health; Organic solvents; Xylene.	
Resumo: 	The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technicians were sampled at two moments: Monday and Friday. DNA damage and cytotoxicity were evaluated using the Comet Assay and the Buccal Micronucleus Cytome assay. Fifteen subjects (83.5%) of the exposed group to solvents complained about some symptom probably related to contact with vapours of organic solvents. DNA damage in the exposed group to solvents was nearly 2-fold higher on Friday than on Monday, and in both moments the individuals of this group showed higher levels of DNA damage in relation to controls. No statistical difference was detected in buccal cell micronucleus frequency between the laboratory technicians and the control group. However, in the analysis performed on Friday, technicians presented higher frequency (about 3-fold) of karyolytic and apoptotic-like cells (karyorrhectic and pyknotic) in relation to control group. Considering the damage frequency and the working time, a positive correlation was found in the exposed group to solvents (r=0.468; p=0.05). The results suggest that pathology laboratory workers inappropriately exposed to organic solvents have increased levels of DNA damage.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100227	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201620150194	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.88 n.1 2016	
Fonte: 	info:eu-repo/semantics/openAccess