Data/hora: 02/06/2024 11:13:26

Provedor de dados: | 42
País: | Brazil
Título: | Two fast screening methods (GC-MS and TLC-ChEI assay) for rapid evaluation of potential anticholinesterasic indole alkaloids in complex mixtures
Autores: | Vieira,Ivo J.C.; Medeiros,Walter L.B.; Monnerat,Cecilia S.; Souza,Jucimar J.; Mathias,Leda; Braz-Filho,Raimundo; Pinto,Angelo C.; Sousa,Priscila M.; Rezende,Claudia M.; Epifanio,Rosângela De A.
Data: | 2008-09-01
Ano: | 2008
Palavras-chave: | Tabernaemontana; Indole alkaloids; Cholinesterase inhibitor; Thin-layer chromatography assay; Ellman´s method; GC-MS.
Resumo: | The pharmacotherapyfor Alzheimer's disease (AD) includes the use of acetylcholinesterase inhibitors (AChEI). Recent investigations for novel AD therapeutic agents from plants suggested that Tabernaemontana genus is a promising source of novel anticholinesterasic indole alkaloids. In this work two fast screening techniques were combined in order to easily identify novel cholinesterase inhibitors (ChEI). Gas chromatography-mass spectrometry (GC-MS) of the less polar alkaloidic fractions obtained from the acid-base extraction of the stalk of T. laeta revealed thirteen monoindole alkaloids, four of them confirmed by co-injection with previously isolated alkaloids. The others were tentatively identified by mass fragmentation analysis. By gas chromatography with flame ionization detection (GC-FID) and using isatin as internal standard, affinisine and voachalotine were determined as major compounds. These fractions and fourteen previously isolated alkaloids, obtained from root bark of T. laeta and T. hystrix were investigated for acetyl (AChE) and butyrylcholinesterase (BuChE) inhibitory activities by the modified Ellman's method in thin layer chromatography(TLC-ChEI). Results showed selective inhibition of the alkaloids heyneanine and Nb-methylvoachalotine for BuChE, and 19-epi-isovoacristine for AChE, whereas olivacine, affinisine, ibogamine, affinine, conodurine and hystrixnineinhibited both enzymes. In addition to confirming that monoterpenoid indole alkaloids can be novel therapeutic agents for AD, this is the first report of the ChEI activity of olivacine, a pyridocarbazole alkaloid.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000300003
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652008000300003
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.80 n.3 2008
Fonte: | info:eu-repo/semantics/openAccess