Data/hora: 16/06/2024 10:24:18

Provedor de dados: | 42
País: | Brazil
Título: | Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation
Autores: | Rainho,Claudia R. de; Kaezer,Andréa; Aiub,Claudia A.F.; Felzenszwalb,Israel.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Allium cepa; Genotoxicity; Mutagenicity; N-nitrosodiethylaine; Tradescantia pallida var. purpurea.
Resumo: | N-nitroso compounds, such as N-nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH) using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM) genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water) and positive control maleic hydrazide (MH 30mg/mL). After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI) and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM), showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dose-dependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400015
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652010000400015
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.82 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess