Data/hora: 10/06/2024 05:33:25

Provedor de dados: | 42
País: | Brazil
Título: | Diosmin induces caspase-dependent apoptosis in human glioblastoma cells
Autores: | SOARES,JULIANA M.; FARIA,BRUNA M. DE; ASCARI,LUCAS M.; ALVES-LEON,SONIZA V.; SOUZA,JORGE M. DE; SOARES,ANTONIO G.; CORDEIRO,YRAIMA; ROMÃO,LUCIANA F.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Apoptosis; Diosmin; Flavonoid; Glioblastoma.
Resumo: | Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in GBM95 and GBM02 cells, making them smaller and more polygonal. Diosmin did not inhibit GBM95 and GBM02 cell proliferation, but it caused DNA fragmentation, as verified by the TUNEL assay, and increased cleaved caspase-3 expression in these cells. In summary, diosmin is able to induce caspase-dependent apoptosis specifically in tumor cells and, therefore, could be considered a promising therapeutic compound against glioblastoma.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700701
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201920191031
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.91 n.4 2019
Fonte: | info:eu-repo/semantics/openAccess