Data/hora: 09/06/2024 03:33:49

Provedor de dados: | 42
País: | Brazil
Título: | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
Autores: | DEMARCHI,GRASIELA; STEFENON,VALDIR M.; STEINER,NEUSA; VIEIRA,FELIPE N.; VESCO,LIRIO L. DAL; GUERRA,MIGUEL P.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Cryopreservation; Ex situ conservation; In vitro culture; Plant germplasm conservation.
Resumo: | This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201420130405
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.86 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess