Data/hora: 08/06/2024 11:38:06

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Modification of the Alere GIARDIA Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats	
Autores: 	COSTA,VIVIANE A.N.; BRENER,BEATRIZ; FONSECA,ANA BEATRIZ M.; SUDRÉ,ADRIANA P.	
Data: 	2018-03-01	
Ano: 	2018	
Palavras-chave: 	Cats; Diagnosis; Dogs; Giardia duodenalis; Immunochromatography.	
Resumo: 	ABSTRACT Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into three groups according to the time of storage in a freezer: (a) ≤ 1 year (n=37); (b) &gt; 1 year and ≤ 3 years (n=39); (c) &gt; 10 years (max. 13 years) (n=24). The results obtained by the modified immunochromatography assay demonstrates a higher sensitivity of this technique when compared with microscopy, regardless of the frozen storage time. These results allow for the use of this methodology in a greater scope of analysis, especially in frozen fecal sediment triage in sample collections, enabling epidemiological and comparative analysis along different decades.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100479	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201820160675	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.90 n.1 2018	
Fonte: 	info:eu-repo/semantics/openAccess