Data/hora: 09/06/2024 06:05:58

Provedor de dados: | Anais da Academia Brasileira de Ciências
País: | Brazil
Título: | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols
Autores: | GOLUNSKI,SIMONE; SILVA,MARCELI F.; MARQUES,CAMILA T.; ROSSETO,VANUSA; KAIZER,ROSILENE R.; MOSSI,ALTEMIR J.; RIGO,DIANE; DALLAGO,ROGÉRIO M.; DI LUCCIO,MARCO; TREICHEL,HELEN.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | Inulinases; Ionic strength; Precipitation; Low cost.
Resumo: | ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L-1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720160367
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess