Data/hora: 09/06/2024 11:31:10

Provedor de dados: | 44
País: | Brazil
Título: | Identificação através de PCR dos genes CryI de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Autores: | Valicente,Fernando H.; Barreto,Marliton R.; Vasconcelos,Maria J. V. de; Figueiredo,José E.F. de; Paiva,Edilson.
Data: | 2000-03-01
Ano: | 2000
Palavras-chave: | Insecta; CryI genes; Biocontrol; Fall armyworm.
Resumo: | Fall armyworm, Spodoptera frugiperda (J. E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to Identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Bioassays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryI general primers and their proteins were analised by SDS-PAGE eletrophoresis. All strains present positive results for cryI genes. To further characterize these strains, specific cryI primers were employed. PCR technique showed that some strains harbour the same cryI genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cryIB and cryID specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000100018
Editor: | Sociedade Entomológica do Brasil
Relação: | 10.1590/S0301-80592000000100018
Formato: | text/html
Fonte: | Anais da Sociedade Entomológica do Brasil  v.29 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess