Data/hora: 17/06/2024 14:54:58

Provedor de dados: | 45
País: | Brazil
Título: | Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
Autores: | Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.
Data: | 2019-06-01
Ano: | 2019
Palavras-chave: | Pig; Embryo; Cryopreservation; Lipids; Apoptosis.
Resumo: | ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P&lt; 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO (50:1) + Na+, PLO (52:3) + Na+ and POO (52:2) + Na. Re-expansion did not differ (P&gt; 0.05) between fresh or vitrified blastocysts at 24 (33.3%; 32.7%) or 48 hours (2.4%; 13.5%). Hatching rates were higher (P&lt; 0.05) for fresh compared to vitrified at 24 (66.7%; 15.4%) and 48 hours (97.6%; 36.0%). BAX was overexpressed (P&lt; 0.05) after vitrification. In conclusion, Piau blastocysts can be cryopreserved by Cryotop. This study also demonstrated that the apoptotic pathway may be responsible for the low efficiency of porcine embryo cryopreservation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-10249
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.71 n.3 2019
Fonte: | info:eu-repo/semantics/openAccess