Data/hora: 13/06/2024 07:11:30

Provedor de dados: 	45	
País: 	Brazil	
Título: 	An alternative method for Staphylococcus aureus DNA isolation	
Autores: 	Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.	
Data: 	2008-04-01	
Ano: 	2008	
Palavras-chave: 	Staphylococcus aureus; Genomic DNA; DNA isolation.	
Resumo: 	This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352008000200004	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.60 n.2 2008	
Fonte: 	info:eu-repo/semantics/openAccess