Data/hora: 03/06/2024 10:57:43

Provedor de dados: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia
País: | Brazil
Título: | An alternative method for Staphylococcus aureus DNA isolation
Autores: | Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.
Data: | 2008-04-01
Ano: | 2008
Palavras-chave: | Staphylococcus aureus; Genomic DNA; DNA isolation.
Resumo: | This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352008000200004
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.60 n.2 2008
Fonte: | info:eu-repo/semantics/openAccess