Data/hora: 03/06/2024 16:21:30

Provedor de dados: | 45
País: | Brazil
Título: | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin
Autores: | Oliveira,K.P.; Reis,A.M.S.; Silva,A.P.; Silva,C.L.R.; Goes,A.M.; Serakides,R.; Ocarino,N.M.
Data: | 2017-11-01
Ano: | 2017
Palavras-chave: | Rat; Osteogenic differentiation; Osteoblasts; Hormone; Adipose tissue.
Resumo: | ABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601573
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-9364
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.69 n.6 2017
Fonte: | info:eu-repo/semantics/openAccess