Data/hora: 11/06/2024 13:21:06

Provedor de dados: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia	
País: 	Brazil	
Título: 	Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus	
Autores: 	Severo,J.S.; Aoki,V.; Santana,A.E.; Mantovani,M.M.; Michalany,N.S.; Larsson Junior,C.E.; Larsson,C.E.	
Data: 	2018-06-01	
Ano: 	2018	
Palavras-chave: 	Immunofluorescence; DIF; IIF; Pemphigus foliaceus.	
Resumo: 	ABSTRACT Pemphigus foliaceus (PF) is the most common autoimmune skin disease in dogs. It is characterized by pustules, erosions, and crusts which occur due to the presence of autoantibodies that target intercellular adhesion. Histopathological examination is considered the gold standard pattern in the diagnosis, but may sometimes be inconclusive, especially when the characteristic findings are not identified. New diagnostic tests are continuously being developed and immunofluorescence assays, could be a valuable alternative diagnostic tool. This study aimed to evaluate the applicability of direct and indirect immunofluorescence (DIF and IIF) tests for the diagnosis of canine PF. Twenty eight dogs were divided into two groups: Group I with 14 dogs with PF and Group II (control) with 14 dogs with Superficial pyoderma (differential diagnoses of PF). All animals were submitted to skin biopsy to histopathological and DIF. Blood samples were collected to assess IIF. Comparing the DIF results against the histopathology test, there was an agreement of 75% (9/12) with a Kappa index of 0.77 (P&lt;0.001). Considering IIF, the agreement was 100% (14/14), with a Kappa index of 1.0 (P&lt;0.001). We conclude that DIF and IIF are highly effective and were useful and effective complementary examination tests for an improvement in the diagnosis of canine PF.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300649	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/1678-4162-9917	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.70 n.3 2018	
Fonte: 	info:eu-repo/semantics/openAccess