Data/hora: 13/06/2024 05:29:00

Provedor de dados: | 45
País: | Brazil
Título: | Manutenção in vitro de células IDE8 em dois tipos de soro bovino
Autores: | Bastos,C.V.; Vasconcelos,M.M.C.; Ribeiro,M.F.B.; Passos,L.M.F.
Data: | 2007-04-01
Ano: | 2007
Palavras-chave: | Bovino; IDE8; Cultivo in vitro; Células de carrapato; Soro fetal bovino.
Resumo: | The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P&lt;0.05) were seen among culture viabilities.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352007000200046
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.59 n.2 2007
Fonte: | info:eu-repo/semantics/openAccess