Data/hora: 09/06/2024 05:50:57

Provedor de dados: | 45
País: | Brazil
Título: | Characterization of gene polymorphisms related to immune system physiology in Mangalarga horses
Autores: | Prioli,R.A.; Curi,R.A.; Chardulo,L.A.; Gomes,V.N.; Prioli,S.M.A.P.; Mota,M.D.S.
Data: | 2012-10-01
Ano: | 2012
Palavras-chave: | Horse; CD14; Cε; SNP; TLR4.
Resumo: | The objectives of this study were to standardize a PCR-RFLP genotyping method for the AY_731081:g.1900T&gt;C SNP of the equine CD14 gene, and to characterize this SNP and two other polymorphisms (AY_005808: c.1530A&gt;G of the TLR4 gene and AX_463789: g.133T&gt;C of the Cε gene) in Mangalarga horses, in order to contribute to future studies investigating the association between DNA markers and traits related to immune system physiology in this breed. A total of 151 Mangalarga horses of both sexes and variable ages, representative of the population of São Paulo State, were used. PCR-RFLP was found to be adequate for genotyping of the AY_731081: g.1900T&gt;C SNP of the equine CD14 gene. However, this polymorphism is probably not present in Mangalarga horses, thus impairing association studies using this marker in the breed. The population genetic parameters obtained for the TLR4 AY_005808:c.1530A&gt;G and Cε AX_463789:g.133T&gt;C polymorphisms suggest the use of these markers in association studies with immune system-related traits in Mangalarga horses.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500030
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352012000500030
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.64 n.5 2012
Fonte: | info:eu-repo/semantics/openAccess